• CAMP-test revers_Arcanobacterium haemolyticum
  • CAMP-test revers_Clostridium perfringens
  • CAMP-test revers_Corynebacterium ulcerans
  • CAMP-test_Actinomyces neuii subsp neuii
  • CAMP-test_Listeria monocytogenes & L. ivanovii
  • CAMP-test_Streptococcus agalactiae
  • Catalase
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  • Dienes phenomenon test_P. mirabilis
  • Fluorescence_Fusobacterium necrophorum, F. nucleatum and F. species
  • Gram staining
  • Granules_Aggregatibacter actinomycetemcomitans
  • Indole test
  • Lipophylic Corynebacteria
  • Medusa head
  • Methylene Blue stain
  • Motility
  • Motility_Listeria monocytogenes_semi solid media
  • Motility_Proteus mirabilis
  • Nitrocefin DrySlide™
  • Optochin test
  • Oxidase test
  • Pyrase test
  • Satellitism test_Haemophilus influenzae
  • Triple Sugar Iron (TSI) Agar

Oxidase test

  • General

    • The oxidase test is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain.

      When present, the cytochrome c oxidase oxidizes the reagent (teramethyl-p-phenylenediamine) to (indophenols) purple color end product.
      When the ezymes is not present, the reagent remains reduced and is colorless.

      Expected results of oxidase test
      1. Positive: Development of dark purple clor (indophenols) within 10 seconds.
      2. Negative: Absence of color.

      Strains may be either oxidase-positive (OX+) or oxidase-negative (OX-).

      OX+ normally means the bacterium contains cytochrome c oxidase and can therefore use oxygen for energy production by converting O2 to H2O2 or H2O with an electron transfer chain. 
      - Pseudomonadaceae are typically OX+
      - Gram negative diplococci Neisseria and Moraxella are OX+
      Many Gram-negative, spiral curved rods which includes Helicobacter pylori, Vibrio cholerae and Campylobacter jejuni are OX+

      Oxidase Variable
      Legionella pneumophila
       may be OX+

      OX− normally means the bacterium does not contain cytochrome c oxidase and, therefore, either cannot use oxygen for energy production with an electyron transfer chain or employs a different cytochrome for transferring electrons to oxygen.
      Enterobacteriaceae are typically OX−

  • History

  • Related

  • References

    • Microbe Online