• CAMP-test revers_Arcanobacterium haemolyticum
  • CAMP-test revers_Clostridium perfringens
  • CAMP-test revers_Corynebacterium ulcerans
  • CAMP-test_Actinomyces neuii subsp neuii
  • CAMP-test_Listeria monocytogenes & L. ivanovii
  • CAMP-test_Streptococcus agalactiae
  • Catalase
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  • Dienes phenomenon test_P. mirabilis
  • Fluorescence_Fusobacterium necrophorum, F. nucleatum and F. species
  • Gram staining
  • Granules_Aggregatibacter actinomycetemcomitans
  • Indole test
  • Lipophylic Corynebacteria
  • Medusa head
  • Methylene Blue stain
  • Motility
  • Motility_Listeria monocytogenes_semi solid media
  • Motility_Proteus mirabilis
  • Nitrocefin DrySlide™
  • Optochin test
  • Oxidase test
  • Pyrase test
  • Satellitism test_Haemophilus influenzae
  • Triple Sugar Iron (TSI) Agar

CAMP-test revers_Clostridium perfringens

  • General

    • ♦ Revers CAMP test

      It can be used for differentiate of Clostridium perfringens from other Clostridium species.

      Here a CAMP-revers positive Streptococcus agalactiae is streaked in the center of sheep blood agar, and a Clostridium perfringens is streaked perpendicular to it.

      Following incubation at 37ºC for 24-48 hours in anaerobic conditions, an "arrowhead" hemolysis is seen between the growth of Clostridium perfringens and Streptococcus agalactiae.
      This is because of alpha toxin produced by Clostridium perfringens interacts with CAMP factor and produce synergistic hemolysis.

  • History

    • The CAMP factor reaction was first described in 1944 by Christie, Atkins and Munch-Peterson and reverse to the synergistic lysis of erythrocytes by the beta hemolysin of Staphylococcus aureus and the extra cellular CFB protein of Streptococcus agalactiae.

  • Related

  • References

    • James Versalovic et al.(2011) Manual of Clinical Microbiology 10th Edition