♦ Revers CAMP test
It can be used for determination of Corynebacterium ulcerans
Here a CAMP-revers positive Corynebacterium ulcerans is streaked in the center of sheep blood agar, and a Staphylococcus aureus is streaked perpendicular to it.
Following incubation at 37ºC for 24-48 hours in aerobic conditions, an "arrowhead" hemolysis is seen between the growth of Corynebacterium ulcerans and Staphylococcus aureus
This is because of alpha toxin produced by Corynebacterium ulcerans interacts with CAMP factor and produce synergistic hemolysis.
The CAMP factor reaction was first described in 1944 by Christie, Atkins and Munch-Peterson and reverse to the synergistic lysis of erythrocytes by the beta hemolysin of Staphylococcus aureus and the extra cellular CFB protein of Streptococcus agalactiae.
James Versalovic et al.(2011) Manual of Clinical Microbiology 10th Edition