Cellulosimicrobium cellulans

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Gram stain Culture

General information
- Formerly known as Oerskovia xanthineolytica
  
  Taxonomy
  Family: Promicromonosporaceae
  Former name: Oerskovia xanthineolytica
  
  Natural habitats
  The environment like the soil, water, decaying plant material.
  
  Clinical significance
  Rarely cause human infections.
  
  Infections mainly occur in immunocompromised patients and very often associated with a foreign body.
Gram stain
- Gram positive rods,
  
  highly branched, pleomorphic and filamentous.
  
  After a long incubation period, they fragment into coccoid rods
  
  Older cultures decolorize easier.
  
  Liquid
  Gram positive rods, pleomorphic, filamentous and branching.
  
  Culture
  Gram positive rods, more coccoid rods
  
  Upon reduction of available food (carbohydrates) the rods are even shorter or coccoid
  
  Kinyoun: negative
Culture characteristics
- Facultative anaerobic
  
  5% CO2 enhanced growth
  
  BA: colonies are not hemolytic, pale yellow to phosphorous yellow, convex, and creamy, they penetrate into the agar (“substrate hyphae”) and are 1-2 mm after 24h.
  After 5-7 days, the colonies became fringed
  
  Substrate hyphae: positive (after 5-7 days)
  
  Aerial hyphae: negative
  
  BBAØ: growth
  
  Some strains are obligate aerobic
Characteristics
References
- James Versalovic et al.(2011) Manual of Clinical Microbiology 10th Edition