Shewanella putrefaciens

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Gram stain Culture

General information
- Taxonomy
  Family: Shewanellaceae
  Formerly: Pseudomonas Malitof apathogenic: S. baltica, S. fidelis, S. frigidimarina, S. profunda
  
  Natural habitat:
  In the environment and in food products, does not belong to the normal flora of the human being.
  - S. algae occurs in the vicinity of salt water
  - S. putrefaciens isolated from fish, chicken, meat, fresh and saltwater.
  
  Clinical significans
  Clinical significance unknown.
  Is often found in mixed cultures. In cellulitis, otitis media, skin and soft tissue infections, peritonitis, respiratory- and urinary tract infections S. algae is most commonly isolated in humans
  > 80% (Note errors) and is considered to be more virulent than S. putrefaciens
Gram stain
- Gram-negative rods.
Culture characteristics
- Obligate Aeroob BA: convex, smooth and sometimes mucoid colonies with yellow / brown - brown pigment and give a green discoloration of the bloodagar.
  - S. algae is always hemolytic (after 48-72 hours), it can be mucoid
  - S. putrefaciens, is usually not- haemolytic and not mucoid
  
  McConkey: growth, non lactose fermenter
  
  BBAØ: no growth
  
  Warning!!!!!! As the two species seem to have different potential for humans, correct identification is important.
Characteristics
References
- James Versalovic et al.(2011) Manual of Clinical Microbiology 10th Edition