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Bacillus subtilus was found in Mars Chocolate Drinks


dangerous bacteria
bacterium causes brief but intens food poisoning

Bacillus subtilis

  • General information


    • B.licheniformis, B. amyloliquefaciens, B.subtilis and B.pumilus comprise the subtilis group, which has been associated with food borne gastro-enteritis

      Taxonomy
      Family: Bacillaceae

      Natural habitats
      Spores are widely distributed in nature.

      Clinical significance
      Is non pathogenic or may occasionally be an opportunistic pathogen for humans.

  • Gram stain

    • Large Gram positive (variable) rods.

      0.7-0.8 x 2.0-3.0 µm

      Spore shape: ellipsoidal / cylindrical
      Spore position: central, paracentral or sometimes subterminal
      Sporangia swelling: negative

  • Culture characteristics

    • Obligate aerobic

      BA: colonial morphology is large, flat, dull, frosted glass appearance, blistering after drying folding, can be β-hemolytic and may become cream colored or brown.

      Colonial morphology is variable, within and between strains and may give the appearance of a mixed culture.

      Pigments varying from cream to yellow, orange, pink and red, to brown or black (media with glucose)

      BBAØ: no growth

      Swarming
      Bacillus subtilis were inoculated at the center of a dish with gelose containing nutrients.
      The bacteria start mass-migrating outwards about twelve hours after inoculation, forming dendrites which reach the border of the dish (diameter 90mm) within a few hours. Two days after inoculation the number of bacteria has increased so much that they diffuse light significantly and appear white.


  • Characteristics

  • References

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