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Pyrase test

  • General

    • Pyrase / BBL™ DrySlide
      Pyrrolidonyl Arylamidase (PYR) test is a rapid test which is used for the presumptive identification of group A beta-hemolytic Streptococci and Enterococci.

      The substrate for the PYR test is L-naphtylamide-ß-naphtylamide wich is hydrolyzed by a specific bacterial enzyme.

      Hydrolysis of the substrate by this enzyme releases free ß-naphtylamide, which is detected by the addition of N,N-dimethylaminocinnamaldehyde.
      This detection reagent couples with the naphthylamide to form a red Schiff base.

      Procedure of PYR test
      The Dryslide contain dried chromogenic substrates for pyrrolidonyl peptidase (PYRase) and beta-gluconidase.
      1. Applie only a few colonies to the test strip
      2. After 2 minutes
      3) Positive: S. pyogenes, E. faecalis and E. faecium

      Negative: No color change or a blue color due to a positive indole reaction.
      Group B Streptococci (Streptococcus agalactiae), Streptococcus mitis, S. bovis, S. equinus, S. milleri.

      Note: A pale pink reaction (weak) is considered negative.

  • History

  • Related

  • References

    • MicrobeOnline
      https://microbeonline.com/pyrrolidonyl-arylamidase-pyr-test-principle-procedure-results/