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Pseudomonas aeruginosa

  • General information

    • the following information is not yet verified
      Taxonomy
      Family: Pseudomonadaceae

      Natural habitat
      Widely distributed in nature. Isolated from soil, water, plants and clinical human specimens.
      It is also found in most man-made environments throughout the world.
      Because they survive in a moist environment, they can cause problems in a hospital.

      Pseudomonas found in all types of aqueous solutions, including disinfectants, soap, eye drops, dialysis fluid, sink etc

      Clinical significance
      Is the most important pathogen of the genus Pseudomonas. They cause a broad spectrum of infections, ranging from superficial skin lesions to sepsis.
      Infections occur mainly in patients with burns, cystic fibrosis, acute leukemia, organ transplants, otitis externa ("swimmer 's ear") and intravenous drug users.
      They also cause urinary tract-, lower respiratory- and eye infections.

  • Gram stain

    • the following information is not yet verified
      Gram-negative rods.

      0.5-0.8 by 1.5-3.0 µm

  • Culture characteristics

    • the following information is not yet verified

      Obligate Aerobic

      3 colony types:
      1) small, rough, wrinkled
      2) large, flat and spreading, serrated edge and a metallic sheen (associated with autolysis of the colony)
      3) mucoid, sphere, smooth surface

      Often they are ß-hemolytic.
      Colonies tend to swarm.

      They produce a blue-green metallic pigment (pyocyanine).

      The blue-green color is a mixture of pyocyanine and pyoverdine.

      Other colors pigment:
      - yellow green - pyoverdine (Fluorescens group)
      - reddish brown - pyorubrine
      - brown black - pyomelanine

      Colonies and surrounding medium have this color.

      Mucoid isolates from CF(often poor prognosis) patients may undergo several phenotypic changes, including slow growth, loss of motility, and loss fof pigment production.
      Some strains produce many extracellular polysaccharide slime, which grow as slimy colonies.

      These are often non-pigmented colonies, and often have a slow oxidase reaction.
      These are common in CF patients.

      McConkey: growth, non lactose fermenter
      often with blue or metallic sheen colonies

      BBAØ: no growth

      Odor/smell
      Grape-like odor, caused by the production of pyocyanine

      Bouillon
      In bouillon the P.aeruginosa grows with a fleece on the surface.
      Production of pyocyanin, water-soluble blue pigment of Pseudomonas aeruginosa

  • Characteristics

  • References


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